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Last updated: 2017/1/15
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HSP90AB1 monoclonal antibody, clone 5G4

  • Catalog # : MAB10686
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant HSP90AB1.
  • Immunogen:
  • Recombinant protein corresponding to human HSP90AB1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 84
  • Reactivity:
  • Human, Monkey, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) against Jurkat (1) , A-431 (2) , HeLa (3) , A-549 (4) , HEK293 (5) , K-562 (6) , NIH/3T3 (7) , PC-12 (8) and COS-7 (9) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues (upper) and brain tissues (bottom) using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) .
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) (green) and negative control (purple) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3326
  • Gene Name:
  • HSP90AB1
  • Gene Alias:
  • D6S182,FLJ26984,HSP90-BETA,HSP90B,HSPC2,HSPCB
  • Gene Description:
  • heat shock protein 90kDa alpha (cytosolic), class B member 1
  • Gene Summary:
  • HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000016517,OTTHUMP00000016518,OTTHUMP00000016519,OTTHUMP00000039869,heat shock 90kD protein 1, beta,heat shock 90kDa protein 1, beta,heat shock protein beta
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