HSP90AA1/HSP90AB1 recombinant monoclonal antibody, clone 10D6
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: K562 whole cell lysate, Lane 4: HL-60 whole cell lysate, Lane 5: HepG2 whole cell lysate, Lane 6: A549 whole cell lysate, Lane 7: Jurkat whole cell lysate, Lane 8: PC3 whole cell lysate and Lane 9: rat brain tissue with HSP90AA1/HSP90AB1 recombinant monoclonal antibody, clone 10D6 (Cat # RAB04363).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with HSP90AA1/HSP90AB1 recombinant monoclonal antibody, clone 10D6 (Cat # RAB04363).
Lane 1: rabbit control IgG, Lane 2: RAB04363 precipitates and Lane 3: Input (Hela whole cell lysate).
Flow Cytometry
Flow cytometric analysis of Jurkat cells with HSP90AA1/HSP90AB1 recombinant monoclonal antibody, clone 10D6 (Cat # RAB04363) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HSP90AA1/HSP90AB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HSP90AA1/HSP90AB1.
Theoretical MW (kDa)
Calculated MW: 90 kD
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: K562 whole cell lysate, Lane 4: HL-60 whole cell lysate, Lane 5: HepG2 whole cell lysate, Lane 6: A549 whole cell lysate, Lane 7: Jurkat whole cell lysate, Lane 8: PC3 whole cell lysate and Lane 9: rat brain tissue with HSP90AA1/HSP90AB1 recombinant monoclonal antibody, clone 10D6 (Cat # RAB04363).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with HSP90AA1/HSP90AB1 recombinant monoclonal antibody, clone 10D6 (Cat # RAB04363).
Lane 1: rabbit control IgG, Lane 2: RAB04363 precipitates and Lane 3: Input (Hela whole cell lysate).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells with HSP90AA1/HSP90AB1 recombinant monoclonal antibody, clone 10D6 (Cat # RAB04363) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — HSP90AA1
Entrez GeneID
3320Protein Accession#
P07900;P08238Gene Name
HSP90AA1
Gene Alias
FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2
Gene Description
heat shock protein 90kDa alpha (cytosolic), class A member 1
Omim ID
140571Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha
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Gene Info — HSP90AB1
Entrez GeneID
3326Protein Accession#
P07900;P08238Gene Name
HSP90AB1
Gene Alias
D6S182, FLJ26984, HSP90-BETA, HSP90B, HSPC2, HSPCB
Gene Description
heat shock protein 90kDa alpha (cytosolic), class B member 1
Omim ID
140572Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
OTTHUMP00000016517|OTTHUMP00000016518|OTTHUMP00000016519|OTTHUMP00000039869|heat shock 90kD protein 1, beta|heat shock 90kDa protein 1, beta|heat shock protein beta
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Interactome
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Pathway
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Disease
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