HSP90AA1 recombinant monoclonal antibody, clone 4B5
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More Files
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Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: K562 whole cell lysate, Lane 4: HL-60 whole cell lysate, Lane 5: HepG2 whole cell lysate, Lane 6: A549 whole cell lysate, Lane 7: Jurkat whole cell lysate, Lane 8: PC3 whole cell lysate and Lane 9: rat brain tissue with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364).
Immunohistochemistry
Immunohistochemical staining of human testis tissue with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364) (diluated at 1:80).
Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364) (diluated at 1:80).
Immunofluorescence
Immunofluorescent staining of NIH/3T3 cells with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364).
Lane 1: rabbit control IgG, Lane 2: RAB04364 precipitates and Lane 3: Input (Hela whole cell lysate).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HSP90AA1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HSP90AA1.
Theoretical MW (kDa)
Calculated MW: 85, 9
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: K562 whole cell lysate, Lane 4: HL-60 whole cell lysate, Lane 5: HepG2 whole cell lysate, Lane 6: A549 whole cell lysate, Lane 7: Jurkat whole cell lysate, Lane 8: PC3 whole cell lysate and Lane 9: rat brain tissue with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364).Immunohistochemistry
Immunohistochemical staining of human testis tissue with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364) (diluated at 1:80).Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364) (diluated at 1:80).Immunofluorescence
Immunofluorescent staining of NIH/3T3 cells with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with HSP90AA1 recombinant monoclonal antibody, clone 4B5 (Cat # RAB04364).
Lane 1: rabbit control IgG, Lane 2: RAB04364 precipitates and Lane 3: Input (Hela whole cell lysate).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSP90AA1
Entrez GeneID
3320Protein Accession#
P07900Gene Name
HSP90AA1
Gene Alias
FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2
Gene Description
heat shock protein 90kDa alpha (cytosolic), class A member 1
Omim ID
140571Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha
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Interactome
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Pathway
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Disease
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