HSP90AB1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of PC12 (A), Raw264.7 (B), Hela (C), A549 (D), HL60 (E) whole cell lysates with HSP90AB1 recombinant monoclonal antibody (Cat # RAB02592).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using HSP90AB1 recombinant monoclonal antibody (Cat # RAB02592). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.20). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HSP90AB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human HSP90AB1.
Theoretical MW (kDa)
90
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of HSP90 beta protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PC12 (A), Raw264.7 (B), Hela (C), A549 (D), HL60 (E) whole cell lysates with HSP90AB1 recombinant monoclonal antibody (Cat # RAB02592).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using HSP90AB1 recombinant monoclonal antibody (Cat # RAB02592). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.20). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Gene Info — HSP90AB1
Entrez GeneID
3326Protein Accession#
P08238Gene Name
HSP90AB1
Gene Alias
D6S182, FLJ26984, HSP90-BETA, HSP90B, HSPC2, HSPCB
Gene Description
heat shock protein 90kDa alpha (cytosolic), class B member 1
Omim ID
140572Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
OTTHUMP00000016517|OTTHUMP00000016518|OTTHUMP00000016519|OTTHUMP00000039869|heat shock 90kD protein 1, beta|heat shock 90kDa protein 1, beta|heat shock protein beta
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