GSTP1 monoclonal antibody, clone 3F2C2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GSTP1 cDNA (2) .
Western Blot
Western blot analysis using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) against PC-3 cell lysate (1) and human cerebellum tissue lysate (2) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate tissues using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) with DAB staining.
Immunofluorescence
Confocal immunofluorescence analysis of HepG2 (upper) and L-02 (bottom) cells using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence
Confocal immunofluorescence analysis of PC-3 cells using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) (green) . Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of K-562 cells using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GSTP1.
Immunogen
Recombinant protein corresponding to human GSTP1.
Host
Mouse
Theoretical MW (kDa)
23
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GSTP1 cDNA (2) .Western Blot
Western blot analysis using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) against PC-3 cell lysate (1) and human cerebellum tissue lysate (2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate tissues using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) with DAB staining.Immunofluorescence
Confocal immunofluorescence analysis of HepG2 (upper) and L-02 (bottom) cells using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Immunofluorescence
Confocal immunofluorescence analysis of PC-3 cells using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) (green) . Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of K-562 cells using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) (green) and negative control (purple). -
Gene Info — GSTP1
Entrez GeneID
2950Gene Name
GSTP1
Gene Alias
DFN7, FAEES3, GST3, PI
Gene Description
glutathione S-transferase pi 1
Omim ID
134660Gene Ontology
HyperlinkGene Summary
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq
Other Designations
OTTHUMP00000174659|deafness, X-linked 7|fatty acid ethyl ester synthase III|glutathione transferase
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Interactome
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Pathway
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Disease
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