Mouse monoclonal antibody raised against partial recombinant GSTP1.
Recombinant protein corresponding to human GSTP1.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human prostate tissues using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) with DAB staining.
Confocal immunofluorescence analysis of PC-3 cells using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) (green) . Blue: DRAQ5 fluorescent DNA dye.
Confocal immunofluorescence analysis of HepG2 (upper) and L-02 (bottom) cells using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of K-562 cells using GSTP1 monoclonal antibody, clone 3F2C2 (Cat # MAB10675) (green) and negative control (purple) .
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq