GSTP1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human GSTP1.
Immunogen
Recombinant protein corresponding to human GSTP1.
Sequence
TLGLYGKDQQEAALVDMVNDGVEDLRCKYISLIYTNYEAGKDDYVKALPGQLKPFETLLS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:2500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with GSTP1 polyclonal antibody (Cat # PAB30867).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human esophagus with GSTP1 polyclonal antibody (Cat # PAB30867) shows cytoplasmic positivity in squamous epithelial cells.Immunofluorescence
Immunofluorescent staining of U-2 OS with GSTP1 polyclonal antibody (Cat # PAB30867) (Green) shows positivity in cytoplasm and mitochondria. -
Gene Info — GSTP1
Entrez GeneID
2950Protein Accession#
P09211Gene Name
GSTP1
Gene Alias
DFN7, FAEES3, GST3, PI
Gene Description
glutathione S-transferase pi 1
Omim ID
134660Gene Ontology
HyperlinkGene Summary
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq
Other Designations
OTTHUMP00000174659|deafness, X-linked 7|fatty acid ethyl ester synthase III|glutathione transferase
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Interactome
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Pathway
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Disease
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Publication Reference
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Breast Cancer Proteomics - Differences in Protein Expression between Estrogen Receptor-Positive and -Negative Tumors Identified by Tandem Mass Tag Technology.
Ruckhäberle E, Karn T, Hanker L, Schwarz J, Schulz-Knappe P, Kuhn K, Böhm G, Selzer S, Erhard N, Engels K, Holtrich U, Kaufmann M, Rody A.
Breast Care 2010 Mar; 5(1):7.
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Breast Cancer Proteomics - Differences in Protein Expression between Estrogen Receptor-Positive and -Negative Tumors Identified by Tandem Mass Tag Technology.
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