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Last updated: 2016/12/11
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UBE2I monoclonal antibody, clone 1B10

  • Catalog # : MAB10344
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant UBE2I.
  • Immunogen:
  • Recombinant protein corresponding to human UBE2I.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 18
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) against HeLa (1), HepG2 (2), and COS-7 (3) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) against recombinant human UBE2I protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue (A) and bladder cancer tissue (B) using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HepG2 cells using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7329
  • Gene Name:
  • UBE2I
  • Gene Alias:
  • C358B7.1,P18,UBC9
  • Gene Description:
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • Gene Summary:
  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • SUMO-1-protein ligase,ubiquitin carrier protein,ubiquitin conjugating enzyme 9,ubiquitin-conjugating enzyme E2I,ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9),ubiquitin-conjugating enzyme UbcE2A,ubiquitin-like protein SUMO-1 conjugating enzym
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