RPS6KA3 monoclonal antibody (M01), clone 2G10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RPS6KA3.
Immunogen
RPS6KA3 (NP_004577, 2 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQTEEVSIKEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKISGSDARQ
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.08 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RPS6KA3 monoclonal antibody (M01), clone 2G10. Western Blot analysis of RPS6KA3 expression in HepG2 ( Cat # L019V1 ).Western Blot (Recombinant protein)
ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MAPK3 and RPS6KA3. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to RPS6KA3 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RPS6KA3
Entrez GeneID
6197GeneBank Accession#
NM_004586Protein Accession#
NP_004577Gene Name
RPS6KA3
Gene Alias
CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
Other Designations
OTTHUMP00000023036|insulin-stimulated protein kinase 1|mental retardation, X-linked 19|ribosomal protein S6 kinase, 90kD, polypeptide 3
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Interactome
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Pathway
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Disease
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