RPS6KA3 monoclonal antibody, clone 4E10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) against HeLa (1), MCF-7 (2), and HepG2 (3) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) against recombinant human RPS6KA3 protein.
Immunofluorescence
Immunofluorescence analysis of HepG2 cells using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant RPS6KA3.
Immunogen
Recombinant protein corresponding to human RPS6KA3.
Host
Mouse
Theoretical MW (kDa)
80
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) against HeLa (1), MCF-7 (2), and HepG2 (3) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) against recombinant human RPS6KA3 protein.Immunofluorescence
Immunofluorescence analysis of HepG2 cells using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cells using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) (green) and negative control (red). -
Gene Info — RPS6KA3
Entrez GeneID
6197Gene Name
RPS6KA3
Gene Alias
CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
Other Designations
OTTHUMP00000023036|insulin-stimulated protein kinase 1|mental retardation, X-linked 19|ribosomal protein S6 kinase, 90kD, polypeptide 3
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Interactome
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Pathway
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Disease
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