RPS6KA3 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CHOK1 (A), Ramos (B) whole cell lysates with RPS6KA3 recombinant monoclonal antibody (Cat # RAB02608).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using RPS6KA3 recombinant monoclonal antibody (Cat # RAB02608). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.98). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RPS6KA3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human RPS6KA3.
Theoretical MW (kDa)
84
Reactivity
Hamster, Human
Specificity
Recognizes endogenous levels of RSK2 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CHOK1 (A), Ramos (B) whole cell lysates with RPS6KA3 recombinant monoclonal antibody (Cat # RAB02608).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using RPS6KA3 recombinant monoclonal antibody (Cat # RAB02608). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.98). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — RPS6KA3
Entrez GeneID
6197Protein Accession#
P51812Gene Name
RPS6KA3
Gene Alias
CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
Other Designations
OTTHUMP00000023036|insulin-stimulated protein kinase 1|mental retardation, X-linked 19|ribosomal protein S6 kinase, 90kD, polypeptide 3
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Interactome
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Pathway
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Disease
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