SMAD5 monoclonal antibody (M01), clone 2D7
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SMAD5.
Immunogen
SMAD5 (AAH09682, 105 a.a. ~ 182 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KPLDICEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHNEFNPQHSLLVQFRNLSHNEPHMPQNATFPDSFHQPNN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (34.21 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SMAD5 monoclonal antibody (M01), clone 2D7. Western Blot analysis of SMAD5 expression in K-562 ( Cat # L009V1 ).Western Blot (Cell lysate)
SMAD5 monoclonal antibody (M01), clone 2D7 Western Blot analysis of SMAD5 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Cell lysate)
SMAD5 monoclonal antibody (M01), clone 2D7. Western Blot analysis of SMAD5 expression in Jurkat ( Cat # L017V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SMAD5 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — SMAD5
Entrez GeneID
4090GeneBank Accession#
BC009682Protein Accession#
AAH09682Gene Name
SMAD5
Gene Alias
DKFZp781C1895, DKFZp781O1323, Dwfc, JV5-1, MADH5
Gene Description
SMAD family member 5
Omim ID
603110Gene Ontology
HyperlinkGene Summary
mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD
Other Designations
MAD homolog 5|MAD, mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD, mothers against DPP homolog 5|mothers against decapentaplegic homolog 5|mothers against decapentaplegic, drosophila, homolog of, 5
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Interactome
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Pathway
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Disease
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