SMAD5 monoclonal antibody, clone 3H9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) against HeLa (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K-562 (5) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and lung cancer tissue (B) using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SMAD5.
Immunogen
Recombinant protein corresponding to human SMAD5.
Host
Mouse
Theoretical MW (kDa)
52
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) against HeLa (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K-562 (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and lung cancer tissue (B) using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green) and negative control (purple). -
Gene Info — SMAD5
Entrez GeneID
4090Gene Name
SMAD5
Gene Alias
DKFZp781C1895, DKFZp781O1323, Dwfc, JV5-1, MADH5
Gene Description
SMAD family member 5
Omim ID
603110Gene Ontology
HyperlinkGene Summary
mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD
Other Designations
MAD homolog 5|MAD, mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD, mothers against DPP homolog 5|mothers against decapentaplegic homolog 5|mothers against decapentaplegic, drosophila, homolog of, 5
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Interactome
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Pathway
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Disease
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