SMAD5 (phospho-S463/465) recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The brain tissue lysate of Mouse Lane2:The brain tissue lysate of Rat with SMAD5 (phospho-S463/465) recombinant monoclonal antibody (Cat # RAB02723) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SMAD5 (phospho-S463/465) recombinant monoclonal antibody (Cat # RAB02723). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HepG2 cells using SMAD5 (phospho-S463/465) recombinant monoclonal antibody (Cat # RAB02723)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against SMAD5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant SMAD5.
Theoretical MW (kDa)
58
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of human Smad 5 protein only when phosphorylated at Ser463 and Ser465.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The brain tissue lysate of Mouse Lane2:The brain tissue lysate of Rat with SMAD5 (phospho-S463/465) recombinant monoclonal antibody (Cat # RAB02723) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SMAD5 (phospho-S463/465) recombinant monoclonal antibody (Cat # RAB02723). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HepG2 cells using SMAD5 (phospho-S463/465) recombinant monoclonal antibody (Cat # RAB02723)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS. -
Gene Info — SMAD5
Entrez GeneID
4090Protein Accession#
Q99717Gene Name
SMAD5
Gene Alias
DKFZp781C1895, DKFZp781O1323, Dwfc, JV5-1, MADH5
Gene Description
SMAD family member 5
Omim ID
603110Gene Ontology
HyperlinkGene Summary
mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD
Other Designations
MAD homolog 5|MAD, mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD, mothers against DPP homolog 5|mothers against decapentaplegic homolog 5|mothers against decapentaplegic, drosophila, homolog of, 5
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Interactome
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Pathway
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Disease
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