SMAD5 recombinant monoclonal antibody, clone R07-2A5
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1: C6 and Lane2: Hela lysates with SMAD5 recombinant monoclonal antibody, clone R07-2A5 (Cat # RAB01906).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with SMAD5 recombinant monoclonal antibody, clone R07-2A5 (Cat # RAB01906). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SMAD5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human SMAD5.
Theoretical MW (kDa)
Calculated MW: 52 kD
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1: C6 and Lane2: Hela lysates with SMAD5 recombinant monoclonal antibody, clone R07-2A5 (Cat # RAB01906).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with SMAD5 recombinant monoclonal antibody, clone R07-2A5 (Cat # RAB01906). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunofluorescence
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Gene Info — SMAD5
Entrez GeneID
4090Protein Accession#
Q99717Gene Name
SMAD5
Gene Alias
DKFZp781C1895, DKFZp781O1323, Dwfc, JV5-1, MADH5
Gene Description
SMAD family member 5
Omim ID
603110Gene Ontology
HyperlinkGene Summary
mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD
Other Designations
MAD homolog 5|MAD, mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD, mothers against DPP homolog 5|mothers against decapentaplegic homolog 5|mothers against decapentaplegic, drosophila, homolog of, 5
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Interactome
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Pathway
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Disease
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