GSTP1 monoclonal antibody (M01), clone 2G6-F6
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant GSTP1.
Immunogen
GSTP1 (AAH10915, 1 a.a. ~ 210 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPEFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYVSLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (85)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (48.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GSTP1 expression in transfected 293T cell line by GSTP1 monoclonal antibody (M01), clone 2G6-F6.
Lane 1: GSTP1 transfected lysate (Predicted MW: 23.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of GSTP1 transfected lysate using anti-GSTP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GSTP1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GSTP1 is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to GSTP1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — GSTP1
Entrez GeneID
2950GeneBank Accession#
BC010915Protein Accession#
AAH10915Gene Name
GSTP1
Gene Alias
DFN7, FAEES3, GST3, PI
Gene Description
glutathione S-transferase pi 1
Omim ID
134660Gene Ontology
HyperlinkGene Summary
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq
Other Designations
OTTHUMP00000174659|deafness, X-linked 7|fatty acid ethyl ester synthase III|glutathione transferase
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Polymorphism of the glutathione S-transferase P1 gene (GST-pi) in breast carcinoma.
Khabaz MN.
Polish Journal of Pathology 2014 Jun; 65(2):141.
Application:IHC-P, Human, Breast.
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Endothelial cell dysfunction and cytoskeletal changes associated with repression of p16(INK4a) during immortalization.
Kan CY, Wen VW, Pasquier E, Jankowski K, Chang M, Richards LA, Kavallaris M, MacKenzie KL.
Oncogene 2012 Nov; 31(46):4815.
Application:WB-Ce, Human, Telomerase-immortalized bone marrow endothelial cells.
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Polymorphism of the glutathione S-transferase P1 gene (GST-pi) in breast carcinoma.
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