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BIOtech
BULK-new

HSPB1 polyclonal antibody

  • Catalog # : PAB2048
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of HSPB1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S82 of human HSPB1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-100)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of HSPB1 (arrow) using HSPB1 polyclonal antibody (Cat # PAB2048). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 polyclonal antibody (Cat # PAB2048) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
    This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3315
  • Protein Accession#:
  • NP_001531;P04792
  • Gene Name:
  • HSPB1
  • Gene Alias:
  • CMT2F,DKFZp586P1322,HMN2B,HS.76067,HSP27,HSP28,Hsp25,SRP27
  • Gene Description:
  • heat shock 27kDa protein 1
  • Gene Summary:
  • The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000024846,estrogen-regulated 24 kDa protein,heat shock 27kD protein 1,heat shock protein beta-1,stress-responsive protein 27
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