HSPB1 (phospho S78) recombinant monoclonal antibody, clone 2D8
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:A549 whole cell lysate (not treated) and Lane 2: A549 whole cell lysate (treated with EGF 100ng/ml/20mins) with HSPB1 (phospho S78) recombinant monoclonal antibody, clone 2D8 (Cat # RAB04275).
Immunohistochemistry
Immunohistochemical staining of human lung cancer with HSPB1 (phospho S78) recombinant monoclonal antibody, clone 2D8 (Cat # RAB04275) (diluated at 1:100)
Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with HSPB1 (phospho S78) recombinant monoclonal antibody, clone 2D8 (Cat # RAB04275) (diluated at 1:100).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HSPB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S78 of human HSPB1.
Theoretical MW (kDa)
Calculated MW: 27 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:A549 whole cell lysate (not treated) and Lane 2: A549 whole cell lysate (treated with EGF 100ng/ml/20mins) with HSPB1 (phospho S78) recombinant monoclonal antibody, clone 2D8 (Cat # RAB04275).Immunohistochemistry
Immunohistochemical staining of human lung cancer with HSPB1 (phospho S78) recombinant monoclonal antibody, clone 2D8 (Cat # RAB04275) (diluated at 1:100)Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with HSPB1 (phospho S78) recombinant monoclonal antibody, clone 2D8 (Cat # RAB04275) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSPB1
Entrez GeneID
3315Protein Accession#
P04792Gene Name
HSPB1
Gene Alias
CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Gene Description
heat shock 27kDa protein 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq
Other Designations
OTTHUMP00000024846|estrogen-regulated 24 kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27
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Interactome
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Pathway
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Disease
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