Products

Western blot analysis of lane 1: Hela (0.03 ug/mL), Lane 2: MCF-7 (0.001 ug/mL), Lane 3: Caco-2 (0.1 ug/mL) and Lane 4: A431 (0.03 ug/mL) with HSPB1 recombinant monoclonal antibody, clone SAIC-23C-35 (Cat # RAB03542).
Western Blot analysis the tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots probed with the chimeric r version of RAB03542 before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescent staining of Hela cells with HSPB1 recombinant monoclonal antibody, clone SAIC-23C-35 (Cat # RAB03542).
Immunofluorescence analysis of paraformaldehyde fixed Hela cells permeabilized with 0.15% Triton and stained with the chimeric r version of RAB03542 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03542
(B) DAPI
(C) Merged channels
(D) Isotype control

Flow cytometric analysis of Hela cells with HSPB1 recombinant monoclonal antibody, clone SAIC-23C-35 (Cat # RAB03542).
Paraformaldehyde fixed Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03542 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.