HSPB1 recombinant monoclonal antibody, clone 10C11
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More Files
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Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: PC3 whole cell lysate, Lane 3: 293T whole cell lysate, Lane 4: L02 whole cell lysate, Lane 5: HT-29 whole cell lysate and Lane 6: K562 whole cell lysate with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369).
Immunohistochemistry
Immunohistochemical staining of human cervical cancer with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369) (diluated at 1:61.9).
Immunohistochemistry
Immunohistochemical staining of human pancreatic tissue with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369) (diluated at 1:61.9).
Immunofluorescence
Immunofluorescent staining of MCF-7 cells with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369) (diluated at 1:20). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369).
Lane 1: rabbit control IgG, Lane 2: RAB04369 precipitates and Lane 3: Input (Hela whole cell lysate).
Flow Cytometry
Flow cytometric analysis of HeLa cells with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HSPB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HSPB1.
Theoretical MW (kDa)
Calculated MW: 23 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: PC3 whole cell lysate, Lane 3: 293T whole cell lysate, Lane 4: L02 whole cell lysate, Lane 5: HT-29 whole cell lysate and Lane 6: K562 whole cell lysate with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369).Immunohistochemistry
Immunohistochemical staining of human cervical cancer with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369) (diluated at 1:61.9).Immunohistochemistry
Immunohistochemical staining of human pancreatic tissue with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369) (diluated at 1:61.9).Immunofluorescence
Immunofluorescent staining of MCF-7 cells with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369) (diluated at 1:20). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369).
Lane 1: rabbit control IgG, Lane 2: RAB04369 precipitates and Lane 3: Input (Hela whole cell lysate).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with HSPB1 recombinant monoclonal antibody, clone 10C11 (Cat # RAB04369) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — HSPB1
Entrez GeneID
3315Protein Accession#
P04792Gene Name
HSPB1
Gene Alias
CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Gene Description
heat shock 27kDa protein 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq
Other Designations
OTTHUMP00000024846|estrogen-regulated 24 kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27
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Interactome
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Pathway
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Disease
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