EIF4EBP1 (phospho S65) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of EIF4EBP1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S65 of human EIF4EBP1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of 4EBP1 only when phosphorylated at serine 65.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EIF4EBP1 (phospho S65) polyclonal antibody (Cat # PAB16946).Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF4EBP1
Entrez GeneID
1978Protein Accession#
Q13541Gene Name
EIF4EBP1
Gene Alias
4E-BP1, 4EBP1, BP-1, MGC4316, PHAS-I
Gene Description
eukaryotic translation initiation factor 4E binding protein 1
Omim ID
602223Gene Ontology
HyperlinkGene Summary
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq
Other Designations
eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Cinobufagin Triggers Defects in Spindle Formation and Cap-Dependent Translation in Liver Cancer Cells by Inhibiting the AURKA-mTOR-eIF4E Axis.
Xiaohan Jin, Jiabao Wang, Shuang Zou, Ruicheng Xu, Jin Cao, Yan Zhang, Jia Guo, Xiaochang Wen, Sanmin Deng, Yupiao Zheng, Yu Zhu, Fengmei Wang, Zhongwei Xu.
The American Journal of Chinese Medicine 2020 Apr; 48(3):651.
Application:WB-Tr, Human, Huh7 cells.
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Activation of the p70 S6 kinase by all-trans-retinoic acid in acute promyelocytic leukemia cells.
Lal L, Li Y, Smith J, Sassano A, Uddin S, Parmar S, Tallman MS, Minucci S, Hay N, Platanias LC.
Blood 2005 Feb; 105(4):1669.
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Hierarchical phosphorylation of the translation inhibitor 4E-BP1.
Gingras AC, Raught B, Gygi SP, Niedzwiecka A, Miron M, Burley SK, Polakiewicz RD, Wyslouch-Cieszynska A, Aebersold R, Sonenberg N.
Genes & Development 2001 Nov; 15(21):2852.
Application:WB-Tr, Human, HEK 293T cells.
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Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin.
Brunn GJ, Hudson CC, Sekulic A, Williams JM, Hosoi H, Houghton PJ, Lawrence JC Jr, Abraham RT.
Science 1997 Jul; 277(5322):99.
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Cinobufagin Triggers Defects in Spindle Formation and Cap-Dependent Translation in Liver Cancer Cells by Inhibiting the AURKA-mTOR-eIF4E Axis.
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