EIF4EBP1 recombinant monoclonal antibody, clone 4EB1T37T46-A5
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More Files
- More Functions
Western Blot
Western blot analysis of K562 cell extract untreated or treated with imatinib using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 1 ng/mL.
Immunohistochemistry
Immunohistochemical staining of rat kidney cells including peptide blocking shows phospho-specificity for 4EB1T37T46-A5 Cat. #2041. Anti-rabbit secondary antibody staining (red) is punctate in the nuclei. Neither control peptide (A) nor non-phospho 4E-BP1 peptide (B) blocks 4EB1T37T46-A5 staining but phospho 4E-BP1 (T37/T46) peptide efficiently blocks signal (C). No signal was observed when the primary antibody is omitted (D). The central object in the field is a glomerulus with surrounding tubules sectioned in various orientations.
Flow Cytometry
Peptide blocking flow cytometric analysis of Jurkat cells LY294002 plus wortmannin plus U0126 LWU) treated cells stained with secondary antibody as negative control (light blue) or treated with LWU (red) or treated with FBS (green) or LUW and blocked with phospho-peptide (black) or FBS and blocked with phospho peptide (gold) or LUW and blocked with non-phospho peptide (dark blue) or FBS and blocked with non-phospho peptide (purple) using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 0.1 ug/mL.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EIF4EBP1.
Antibody Species
Rabbit
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr37/46 of human phospho 4E-BP1.
Reactivity
Human
Form
Liquid
Purification
Protein A+G
Isotype
Rabbit IgG1k
Recommend Usage
Flow Cytometry
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
1X PBS, 0.02% Sodium azide, 50% Glycerol, 0.1% BSA
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of K562 cell extract untreated or treated with imatinib using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 1 ng/mL.Immunohistochemistry
Immunohistochemical staining of rat kidney cells including peptide blocking shows phospho-specificity for 4EB1T37T46-A5 Cat. #2041. Anti-rabbit secondary antibody staining (red) is punctate in the nuclei. Neither control peptide (A) nor non-phospho 4E-BP1 peptide (B) blocks 4EB1T37T46-A5 staining but phospho 4E-BP1 (T37/T46) peptide efficiently blocks signal (C). No signal was observed when the primary antibody is omitted (D). The central object in the field is a glomerulus with surrounding tubules sectioned in various orientations.Flow Cytometry
Peptide blocking flow cytometric analysis of Jurkat cells LY294002 plus wortmannin plus U0126 LWU) treated cells stained with secondary antibody as negative control (light blue) or treated with LWU (red) or treated with FBS (green) or LUW and blocked with phospho-peptide (black) or FBS and blocked with phospho peptide (gold) or LUW and blocked with non-phospho peptide (dark blue) or FBS and blocked with non-phospho peptide (purple) using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 0.1 ug/mL. -
Gene Info — EIF4EBP1
Entrez GeneID
1978Protein Accession#
Q13541Gene Name
EIF4EBP1
Gene Alias
4E-BP1, 4EBP1, BP-1, MGC4316, PHAS-I
Gene Description
eukaryotic translation initiation factor 4E binding protein 1
Omim ID
602223Gene Ontology
HyperlinkGene Summary
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq
Other Designations
eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
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Interactome
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Pathway
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Disease
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