EIF4EBP1 (Phospho T46) recombinant monoclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with 4EBP1 (Phospho T46) recombinant monoclonal antibody (Cat # RAB02454).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using 4EBP1 (Phospho T46) recombinant monoclonal antibody (Cat # RAB02454). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.7). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EIF4EBP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr45 of human eIF4EBP1.
Theoretical MW (kDa)
15
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of 4EBP1 (pT46) protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with 4EBP1 (Phospho T46) recombinant monoclonal antibody (Cat # RAB02454).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using 4EBP1 (Phospho T46) recombinant monoclonal antibody (Cat # RAB02454). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.7). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — EIF4EBP1
Entrez GeneID
1978Protein Accession#
Q13541Gene Name
EIF4EBP1
Gene Alias
4E-BP1, 4EBP1, BP-1, MGC4316, PHAS-I
Gene Description
eukaryotic translation initiation factor 4E binding protein 1
Omim ID
602223Gene Ontology
HyperlinkGene Summary
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq
Other Designations
eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
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