BCL2 monoclonal antibody, clone AT1B5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NIH/3T3 cell lysates (35 ug) with BCL2 monoclonal antibody, clone AT1B5 (Cat # MAB9719) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence
Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with BCL2 monoclonal antibody, clone AT1B5 (Cat # MAB9719) at 1 : 500 dilution with Texas Red (Red).
Flow Cytometry
Flow cytometry analysis of HeLa cells, staining at 2-5 ug for 1x106cells.The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant BCL2.
Immunogen
Recombinant protein corresponding to amino acids 1-211 of human Bcl-2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NIH/3T3 cell lysates (35 ug) with BCL2 monoclonal antibody, clone AT1B5 (Cat # MAB9719) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence
Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with BCL2 monoclonal antibody, clone AT1B5 (Cat # MAB9719) at 1 : 500 dilution with Texas Red (Red).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of HeLa cells, staining at 2-5 ug for 1x106cells.The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — BCL2
Entrez GeneID
596Protein Accession#
NP_000624Gene Name
BCL2
Gene Alias
Bcl-2
Gene Description
B-cell CLL/lymphoma 2
Omim ID
151430Gene Ontology
HyperlinkGene Summary
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
Other Designations
B-cell lymphoma protein 2|OTTHUMP00000163680
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Interactome
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Pathway
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Disease
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