GSK3B monoclonal antibody, clone M131
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant GSK3B.
Immunogen
Recombinant protein corresponding to N-terminus of human GSK3B.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This sequence is highly conserved in rat and mouse GSK-3b, but has low homology to GSK-3a.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:4000)
Immunocytochemistry (1:1000)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (Lane 1) or treated with pervanadate (1 mM) for 30 minutes (Lane 2). The blot was probed with GSK3B monoclonal antibody, clone M131 (Cat # MAB1372).Enzyme-linked Immunoabsorbent Assay
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Gene Info — GSK3B
Entrez GeneID
2932Protein Accession#
P49841Gene Name
GSK3B
Gene Alias
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Gene Description
glycogen synthase kinase 3 beta
Omim ID
605004Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
Other Designations
GSK3beta isoform|glycogen synthase kinase-3 beta
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Glycogen synthase kinase-3beta is tyrosine-phosphorylated by MEK1 in human skin fibroblasts.
Takahashi-Yanaga F, Shiraishi F, Hirata M, Miwa Y, Morimoto S, Sasaguri T.
Biochemical and Biophysical Research Communications 2004 Apr; 316(2):411.
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Glycogen synthase kinase-3beta suppression eliminates tumor necrosis factor-related apoptosis-inducing ligand resistance in prostate cancer.
Liao X, Zhang L, Thrasher JB, Du J, Li B.
Molecular Cancer Therapeutics 2003 Nov; 2(11):1215.
Application:WB-Tr, Human, PC-3 cells.
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Expression and characterization of GSK-3 mutants and their effect on beta-catenin phosphorylation in intact cells.
Hagen T, Di Daniel E, Culbert AA, Reith AD.
The Journal of Biological Chemistry 2002 Jun; 277(26):23330.
Application:WB-Tr, Human, HEK 293T cells.
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Glycogen synthase kinase-3beta is tyrosine-phosphorylated by MEK1 in human skin fibroblasts.
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