Mouse monoclonal antibody raised against partial recombinant JMJD2A.
Immunogen:
Recombinant protein corresponding to human JMJD2A.
Host:
Mouse
Theoretical MW (kDa):
120
Reactivity:
Human
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Transfected lysate)
Western blot analysis using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) against HEK293 (1) and JMJD2A-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (upper) and human larynx cancer tissues (bottom) using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq
Other Designations:
OTTHUMP00000008810,jumonji C domain-containing histone demethylase 3A