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JMJD2A monoclonal antibody, clone 5H1

  • Catalog # : MAB10708
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant JMJD2A.
  • Immunogen:
  • Recombinant protein corresponding to human JMJD2A.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 120
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) against HEK293 (1) and JMJD2A-hIgGFc transfected HEK293 (2) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (upper) and human larynx cancer tissues (bottom) using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of NTERA-2 cells using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 9682
  • Gene Name:
  • JMJD2A
  • Gene Alias:
  • JHDM3A,JMJD2,KDM4A,KIAA0677
  • Gene Description:
  • jumonji domain containing 2A
  • Gene Summary:
  • This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000008810,jumonji C domain-containing histone demethylase 3A
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