JMJD2A monoclonal antibody, clone AEHB-11
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human JMJD2A.
Immunogen
A synthetic peptide corresponding to human JMJD2A.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
The antibody reacts with human, mouse JMJD2A, in native form and recombinant. Superfamily members of JMJD2A are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:60)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293 cell lysate; Lane 2: NIH/3T3 cell lysate.Immunohistochemistry
Immunoprecipitation
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Gene Info — JMJD2A
Entrez GeneID
9682Protein Accession#
O75164Gene Name
JMJD2A
Gene Alias
JHDM3A, JMJD2, KDM4A, KIAA0677
Gene Description
jumonji domain containing 2A
Omim ID
609764Gene Ontology
HyperlinkGene Summary
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq
Other Designations
OTTHUMP00000008810|jumonji C domain-containing histone demethylase 3A
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