JMJD2A monoclonal antibody, clone 6E10G4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) JMJD2A-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with JMJD2A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of JMJD2A monoclonal antibody, clone 6E10G4.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human JMJD2A.
Immunogen
Recombinant protein corresponding to amino acid 932-1057 of human JMJD2A from E. coli.
Host
Mouse
Theoretical MW (kDa)
120.6kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) JMJD2A-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with JMJD2A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of JMJD2A monoclonal antibody, clone 6E10G4. -
Gene Info — JMJD2A
Entrez GeneID
9682Gene Name
JMJD2A
Gene Alias
JHDM3A, JMJD2, KDM4A, KIAA0677
Gene Description
jumonji domain containing 2A
Omim ID
609764Gene Ontology
HyperlinkGene Summary
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq
Other Designations
OTTHUMP00000008810|jumonji C domain-containing histone demethylase 3A
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Interactome
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