JMJD2A polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with JMJD2A polyclonal antibody (Cat # PAB20387) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with JMJD2A polyclonal antibody (Cat # PAB20387) shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with JMJD2A polyclonal antibody (Cat # PAB20387) at 1-4 ug/mL dilution shows positivity in nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant JMJD2A.
Immunogen
Recombinant protein corresponding to amino acids of human JMJD2A.
Sequence
DVFVRKFQPERYKLWKAGKDNTVIDHTLPTPEAAEFLKESELPPRAGNEEECPEEDMEGVEDGEEGDLKTSLAKHRIGTKRHRVCLEIPQEVSQSELFPKEDLSSEQYEMTEC
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with JMJD2A polyclonal antibody (Cat # PAB20387) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with JMJD2A polyclonal antibody (Cat # PAB20387) shows strong cytoplasmic positivity in cells in seminiferus ducts.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with JMJD2A polyclonal antibody (Cat # PAB20387) at 1-4 ug/mL dilution shows positivity in nucleoli. -
Gene Info — JMJD2A
Entrez GeneID
9682Protein Accession#
O75164Gene Name
JMJD2A
Gene Alias
JHDM3A, JMJD2, KDM4A, KIAA0677
Gene Description
jumonji domain containing 2A
Omim ID
609764Gene Ontology
HyperlinkGene Summary
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq
Other Designations
OTTHUMP00000008810|jumonji C domain-containing histone demethylase 3A
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