HSPB1 monoclonal antibody, clone 5D7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HSPB1.
Immunogen
Recombinant protein corresponding to human HSPB1.
Host
Mouse
Theoretical MW (kDa)
27
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) against HeLa (1) , A-549 (2) , Jurkat (3) , A-431 (4) , HEK293 (5) , HepG2 (6) and PC-12 (7) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissues (A) , esophageal cancer tissues (B) , breast cancer tissues (C) and cardiac muscle tissues (D) using HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) .Flow Cytometry
Flow cytometric analysis of HepG2 cells using HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) (green) and negative control (purple). -
Gene Info — HSPB1
Entrez GeneID
3315Gene Name
HSPB1
Gene Alias
CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Gene Description
heat shock 27kDa protein 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq
Other Designations
OTTHUMP00000024846|estrogen-regulated 24 kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27
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Interactome
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