GSK3B monoclonal antibody, clone 3D10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) against A-549 (1) , K-562 (2) , PC-12 (3) , NIH/3T3 (4) , and HEK293 (5) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and breast cancer tissues (bottom) using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 (upper) and U251 (bottom) cells using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of HeLa cells using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GSK3B.
Immunogen
Recombinant protein corresponding to human GSK3B.
Host
Mouse
Theoretical MW (kDa)
46
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) against A-549 (1) , K-562 (2) , PC-12 (3) , NIH/3T3 (4) , and HEK293 (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and breast cancer tissues (bottom) using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 (upper) and U251 (bottom) cells using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) (green) and negative control (purple). -
Gene Info — GSK3B
Entrez GeneID
2932Gene Name
GSK3B
Gene Alias
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Gene Description
glycogen synthase kinase 3 beta
Omim ID
605004Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
Other Designations
GSK3beta isoform|glycogen synthase kinase-3 beta
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Interactome
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Pathway
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Disease
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