Mouse monoclonal antibody raised against partial recombinant GSK3B.
Recombinant protein corresponding to human GSK3B.
Theoretical MW (kDa):
Human, Monkey, Mouse, Rat
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate)
Western blot analysis using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) against A-549 (1) , K-562 (2) , PC-12 (3) , NIH/3T3 (4) , and HEK293 (5) cell lysate.
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and breast cancer tissues (bottom) using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) with DAB staining.
Immunofluorescence analysis of NIH/3T3 (upper) and U251 (bottom) cells using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of HeLa cells using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) (green) and negative control (purple) .
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene