CHAT recombinant monoclonal antibody, clone 8E7
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Mouse brain tissue lysate.
Immunohistochemistry
Immunohistochemistry image of CHAT recombinant monoclonal antibody, clone 8E7 diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of SH-SY5Y Cells with CHAT recombinant monoclonal antibody, clone 8E7 at 1:30, counter-stained with DAPI.
Flow Cytometry
Overlay Peak curve showing SH-SY5Y cells stained with CHAT recombinant monoclonal antibody, clone 8E7 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse CHAT.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CHAT.
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Mouse brain tissue lysate.Immunohistochemistry
Immunohistochemistry image of CHAT recombinant monoclonal antibody, clone 8E7 diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of SH-SY5Y Cells with CHAT recombinant monoclonal antibody, clone 8E7 at 1:30, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing SH-SY5Y cells stained with CHAT recombinant monoclonal antibody, clone 8E7 (red line) at 1:50. -
Gene Info — CHAT
Entrez GeneID
1103Protein Accession#
P28329Gene Name
CHAT
Gene Alias
CMS1A, CMS1A2
Gene Description
choline acetyltransferase
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq
Other Designations
OTTHUMP00000019583|OTTHUMP00000019584|acetyl CoA:choline O-acetyltransferase
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Interactome
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Pathway
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Disease
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