CHAT polyclonal antibody
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CHAT polyclonal antibody (Cat # PAB29065) was analyzed by immunocytological staining (at a concentration of 3 ug/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution) as the secondary reagent.
CHAT-positive neurons (red) in the neostriatum of a GFP-expressing transgenic mouse.
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Specification
Product Description
Chicken polyclonal antibody raised against recombinant Human CHAT.
Immunogen
A KLH-conjugated synthetic peptide corresponding to a region of CHAT gene product shared between the human (P_066264, NCBI) and mouse (Q03059, NCBI) sequences.
Host
Chicken
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgY
Quality Control Testing
Immunocytochemistry
CHAT polyclonal antibody (Cat # PAB29065) was analyzed by immunocytological staining (at a concentration of 3 ug/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution) as the secondary reagent.
CHAT-positive neurons (red) in the neostriatum of a GFP-expressing transgenic mouse.Recommend Usage
Immunocytochemistry(1:1000-1:2000)
Immunohistochemistry(1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide)
Storage Instruction
Store at 4°C and avoid from light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunocytochemistry
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Gene Info — CHAT
Entrez GeneID
1103Protein Accession#
P_066264Gene Name
CHAT
Gene Alias
CMS1A, CMS1A2
Gene Description
choline acetyltransferase
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq
Other Designations
OTTHUMP00000019583|OTTHUMP00000019584|acetyl CoA:choline O-acetyltransferase
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Gene Info — Chat
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Interactome
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Pathway
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Disease
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