CHAT polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CHAT.
Immunogen
A synthetic peptide corresponding to human CHAT.
Sequence
CKEKATRPSQGHQP
Host
Goat
Theoretical MW (kDa)
70.4, 82.6, 74.4
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms. NP_066264, NP_066265 and NP_066266 are identical proteins representing isoform 1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.03-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CHAT polyclonal antibody (Cat # PAB6736) (0.03 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CHAT
Entrez GeneID
1103Protein Accession#
NP_065574;NP_066264;NP_066265;NP_066266Gene Name
CHAT
Gene Alias
CMS1A, CMS1A2
Gene Description
choline acetyltransferase
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq
Other Designations
OTTHUMP00000019583|OTTHUMP00000019584|acetyl CoA:choline O-acetyltransferase
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Interactome
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Pathway
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Disease
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Publication Reference
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Regulation of cholinergic gene expression by nerve growth factor depends on the phosphatidylinositol-3'-kinase pathway.
Madziar B, Lopez-Coviella I, Zemelko V, Berse B.
Journal of Neurochemistry 2005 Feb; 92(4):767.
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Regulation of cholinergic gene expression by nerve growth factor depends on the phosphatidylinositol-3'-kinase pathway.
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