CHAT recombinant monoclonal antibody, clone R07-1G8
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More Files
- More Functions
Western Blot
Western blot analysis of Choline Acetyltransferase in rat Brain lysates using human Choline Acetyltransferase recombinant monoclonal antibody, clone R07-1G8 (Cat # RAB01502).
Immunocytochemistry
Immunocytochemistry analysis of Choline Acetyltransferase (green) in SH-SY5Y using human Choline Acetyltransferase recombinant monoclonal antibody, clone R07-1G8 (Cat # RAB01502), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Choline Acetyltransferase.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Choline Acetyltransferase
Theoretical MW (kDa)
Calculated MW: 83 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Choline Acetyltransferase in rat Brain lysates using human Choline Acetyltransferase recombinant monoclonal antibody, clone R07-1G8 (Cat # RAB01502).Immunocytochemistry
Immunocytochemistry analysis of Choline Acetyltransferase (green) in SH-SY5Y using human Choline Acetyltransferase recombinant monoclonal antibody, clone R07-1G8 (Cat # RAB01502), and DAPI(blue).Immunoprecipitation
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Gene Info — CHAT
Entrez GeneID
1103Protein Accession#
P28329Gene Name
CHAT
Gene Alias
CMS1A, CMS1A2
Gene Description
choline acetyltransferase
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq
Other Designations
OTTHUMP00000019583|OTTHUMP00000019584|acetyl CoA:choline O-acetyltransferase
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Interactome
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Pathway
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Disease
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