INSR recombinant monoclonal antibody, clone 4B2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human INSR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human INSR.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescent staining of MCF7 Cells with INSR recombinant monoclonal antibody, clone 4B2 (Cat # RAB07439). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).Enzyme-linked Immunoabsorbent Assay
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Gene Info — INSR
Entrez GeneID
3643Protein Accession#
P06213Gene Name
INSR
Gene Alias
CD220, HHF5
Gene Description
insulin receptor
Gene Ontology
HyperlinkGene Summary
After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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