Mouse IgG(H+L) VHH recombinant monoclonal antibody (Biotin)
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Specification
Product Description
Alpaca VHH recombinant monoclonal antibody raised against mouse IgG(H+L).
The antibody is conjugated with biotin.Antibody Species
Alpaca
Immunogen
Mouse IgG recombinant protein
Reactivity
Mouse
Specificity
Mouse IgG (H&L)
Form
Liquid
Conjugation
Biotin
Purification
Protein A
Isotype
Alpaca IgG2b/2c
Recommend Usage
ELISA (1:10000-1:50000)
Immunoprecipitation (1-2ug)
Surface Plasmon Resonance
Western Blot (1:10000-1:50000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.5 (0.05% sucrose, 0.1% trehalose, 0.01% proclin 300)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. Mouse VHH IgG (H&L) recombinant monoclonal antibody (Biotin) (Cat # RAB07085) was used as the primary antibody at 1 :1000 and streptavidin (HRP) (Cat # RAB07268) as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG or different isotope of mouse IgG. Mouse VHH IgG (H&L) recombinant monoclonal antibody (Biotin) (Cat # RAB07085) was used as the primary antibody at 1:10000 and streptavidin (HRP) (Cat # RAB07268) as the secondary antibody.Surface Plasmon Resonance
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