EIF2AK2 (phospho T446) recombinant monoclonal antibody, clone 2F8
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: Hela whole cell lysate (not treated) with EIF2AK2 (phospho T446) recombinant monoclonal antibody, clone 2F8 (Cat # RAB04235).
Immunohistochemistry
Immunohistochemical staining of human kidney tissue with EIF2AK2 (phospho T446) recombinant monoclonal antibody, clone 2F8 (Cat # RAB04235) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with EIF2AK2 (phospho T446) recombinant monoclonal antibody, clone 2F8 (Cat # RAB04235) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EIF2AK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding T446 of human EIF2AK2.
Theoretical MW (kDa)
Calculated MW: 62 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: Hela whole cell lysate (not treated) with EIF2AK2 (phospho T446) recombinant monoclonal antibody, clone 2F8 (Cat # RAB04235).Immunohistochemistry
Immunohistochemical staining of human kidney tissue with EIF2AK2 (phospho T446) recombinant monoclonal antibody, clone 2F8 (Cat # RAB04235) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with EIF2AK2 (phospho T446) recombinant monoclonal antibody, clone 2F8 (Cat # RAB04235) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF2AK2
Entrez GeneID
5610Protein Accession#
P19525Gene Name
EIF2AK2
Gene Alias
EIF2AK1, MGC126524, PKR, PRKR
Gene Description
eukaryotic translation initiation factor 2-alpha kinase 2
Omim ID
176871Gene Ontology
HyperlinkGene Summary
interferon-inducible double stranded RNA dependent
Other Designations
OTTHUMP00000126954|double stranded RNA activated protein kinase|interferon-inducible elF2alpha kinase|protein kinase, interferon-inducible double stranded RNA dependent
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Interactome
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Disease
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