EIF2AK2 (Phospho T446) recombinant monoclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded rat brain using anti using EIF2AK2 (Phospho T446) recombinant monoclonal antibody (Cat # RAB02636). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation at 1:200 overnight at 4°C.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EIF2AK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against KLH conjugated Synthesised phosphopeptide derived from human EIF2AK2 around the phosphorylation site of Thr446.
Theoretical MW (kDa)
67
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of EIF2AK2 protein only when phosphorylated at Thr446.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.01M TBS, pH7.4 (1% BSA, 0.03% Proclin300 and 50% Glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded rat brain using anti using EIF2AK2 (Phospho T446) recombinant monoclonal antibody (Cat # RAB02636). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation at 1:200 overnight at 4°C. -
Gene Info — EIF2AK2
Entrez GeneID
5610Protein Accession#
Q52M43Gene Name
EIF2AK2
Gene Alias
EIF2AK1, MGC126524, PKR, PRKR
Gene Description
eukaryotic translation initiation factor 2-alpha kinase 2
Omim ID
176871Gene Ontology
HyperlinkGene Summary
interferon-inducible double stranded RNA dependent
Other Designations
OTTHUMP00000126954|double stranded RNA activated protein kinase|interferon-inducible elF2alpha kinase|protein kinase, interferon-inducible double stranded RNA dependent
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Interactome
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Disease
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