EIF2AK2 recombinant monoclonal antibody, clone R08-2C8
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More Files
- More Functions
Western Blot
Western blot analysis of PKR in K562, Hela lysates using human PKR recombinant monoclonal antibody, clone R08-2C8 (Cat # RAB01720).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human Cholangiocarcinoma with PKR recombinant monoclonal antibody, clone R08-2C8 (Cat # RAB01720). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunocytochemistry
Immunocytochemical staining of Hela with PKR recombinant monoclonal antibody, clone R08-2C8 (Cat # RAB01720). (A) PKR (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human EIF2AK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PKR
Theoretical MW (kDa)
Calculated MW: 62 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of PKR in K562, Hela lysates using human PKR recombinant monoclonal antibody, clone R08-2C8 (Cat # RAB01720).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human Cholangiocarcinoma with PKR recombinant monoclonal antibody, clone R08-2C8 (Cat # RAB01720). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemical staining of Hela with PKR recombinant monoclonal antibody, clone R08-2C8 (Cat # RAB01720). (A) PKR (green) and (B) DAPI (blue).Immunoprecipitation
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Gene Info — EIF2AK2
Entrez GeneID
5610Protein Accession#
P19525Gene Name
EIF2AK2
Gene Alias
EIF2AK1, MGC126524, PKR, PRKR
Gene Description
eukaryotic translation initiation factor 2-alpha kinase 2
Omim ID
176871Gene Ontology
HyperlinkGene Summary
interferon-inducible double stranded RNA dependent
Other Designations
OTTHUMP00000126954|double stranded RNA activated protein kinase|interferon-inducible elF2alpha kinase|protein kinase, interferon-inducible double stranded RNA dependent
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Interactome
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Disease
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