EIF2AK2 (phospho T446) recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela treated with Calyculin A and TNF-alpha whole cell lysates Lane 2: Untreated Hela whole cell lysates with EIF2AK2 (phospho T446) recombinant monoclonal antibody (Cat # RAB02735) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using EIF2AK2 (phospho T446) recombinant monoclonal antibody (Cat # RAB02735). Counter stained with hematoxylin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against EIF2AK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant EIF2AK2.
Theoretical MW (kDa)
62
Reactivity
Human
Specificity
This antibody detects endogenous levels of human PKR protein only when phosphorylated at Thr446.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela treated with Calyculin A and TNF-alpha whole cell lysates Lane 2: Untreated Hela whole cell lysates with EIF2AK2 (phospho T446) recombinant monoclonal antibody (Cat # RAB02735) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using EIF2AK2 (phospho T446) recombinant monoclonal antibody (Cat # RAB02735). Counter stained with hematoxylin. -
Gene Info — EIF2AK2
Entrez GeneID
5610Protein Accession#
P19525Gene Name
EIF2AK2
Gene Alias
EIF2AK1, MGC126524, PKR, PRKR
Gene Description
eukaryotic translation initiation factor 2-alpha kinase 2
Omim ID
176871Gene Ontology
HyperlinkGene Summary
interferon-inducible double stranded RNA dependent
Other Designations
OTTHUMP00000126954|double stranded RNA activated protein kinase|interferon-inducible elF2alpha kinase|protein kinase, interferon-inducible double stranded RNA dependent
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Interactome
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Disease
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