RPS6KA1 (phospho T359/S363) recombinant monoclonal antibody, clone 1B6
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More Files
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Western Blot
Western blot analysis of HepG2 whole cell lysate with RPS6KA1 (phospho T359/S363) recombinant monoclonal antibody, clone 1B6 (Cat # RAB04220).
Lane 1: Treated with EGF 100 ng/ml 20mins and Lane 2: Not treated with EGF.
Immunofluorescence
Immunofluorescent staining of Hela cells with RPS6KA1 (phospho T359/S363) recombinant monoclonal antibody, clone 1B6 (Cat # RAB04220) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HeLa whole cell lysate with RPS6KA1 (phospho T359/S363) recombinant monoclonal antibody, clone 1B6 (Cat # RAB04220).
Lane 1: rabbit control IgG, Lane 2: RAB04220 precipitates and Lane 3 : Input (Hela whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RPS6KA1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding T359/S363 of human RPS6KA1.
Theoretical MW (kDa)
Calculated MW: 90 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of HepG2 whole cell lysate with RPS6KA1 (phospho T359/S363) recombinant monoclonal antibody, clone 1B6 (Cat # RAB04220).
Lane 1: Treated with EGF 100 ng/ml 20mins and Lane 2: Not treated with EGF.Immunofluorescence
Immunofluorescent staining of Hela cells with RPS6KA1 (phospho T359/S363) recombinant monoclonal antibody, clone 1B6 (Cat # RAB04220) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HeLa whole cell lysate with RPS6KA1 (phospho T359/S363) recombinant monoclonal antibody, clone 1B6 (Cat # RAB04220).
Lane 1: rabbit control IgG, Lane 2: RAB04220 precipitates and Lane 3 : Input (Hela whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPS6KA1
Entrez GeneID
6195Protein Accession#
Q15418Gene Name
RPS6KA1
Gene Alias
HU-1, MAPKAPK1A, RSK, RSK1
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 1
Omim ID
601684Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000004113|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|ribosomal protein S6 kinase alpha 1|ribosomal protein S6 kinase, 90kD, 1|ribosomal protein S6 kinase, 90kD, polypeptide 1
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Interactome
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Pathway
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Disease
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