RPS6KA1 (phospho T573) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated 293 cells, Lane 2: antigen-specific peptide treated 293 cells with RPS6KA1 (phospho T573) polyclonal antibody (Cat# PAB29263) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with RPS6KA1 (phospho T573) polyclonal antibody (Cat# PAB29263) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RPS6KA1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T573 of human RPS6KA1.
Host
Rabbit
Theoretical MW (kDa)
95
Reactivity
Human, Mouse, Rat
Specificity
RPS6KA1 (phospho T573) polyclonal antibody detects endogenous levels of human RPS6KA1 only when phosphorylated at threonine 573.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated 293 cells, Lane 2: antigen-specific peptide treated 293 cells with RPS6KA1 (phospho T573) polyclonal antibody (Cat# PAB29263) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with RPS6KA1 (phospho T573) polyclonal antibody (Cat# PAB29263) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — RPS6KA1
Entrez GeneID
6195Protein Accession#
Q15418Gene Name
RPS6KA1
Gene Alias
HU-1, MAPKAPK1A, RSK, RSK1
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 1
Omim ID
601684Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000004113|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|ribosomal protein S6 kinase alpha 1|ribosomal protein S6 kinase, 90kD, 1|ribosomal protein S6 kinase, 90kD, polypeptide 1
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Interactome
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Pathway
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Disease
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