RPS6KA1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with RPS6KA1 polyclonal antibody (Cat # PAB30790).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with RPS6KA1 polyclonal antibody (Cat # PAB30790) shows strong cytoplasmic positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of A-431 cells with RPS6KA1 polyclonal antibody (Cat # PAB30790) (Green) shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human RPS6KA1.
Immunogen
Recombinant protein corresponding to human RPS6KA1.
Sequence
KMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL
Host
Rabbit
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with RPS6KA1 polyclonal antibody (Cat # PAB30790).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with RPS6KA1 polyclonal antibody (Cat # PAB30790) shows strong cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of A-431 cells with RPS6KA1 polyclonal antibody (Cat # PAB30790) (Green) shows positivity in cytoplasm. -
Gene Info — RPS6KA1
Entrez GeneID
6195Protein Accession#
Q15418Gene Name
RPS6KA1
Gene Alias
HU-1, MAPKAPK1A, RSK, RSK1
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 1
Omim ID
601684Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000004113|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|ribosomal protein S6 kinase alpha 1|ribosomal protein S6 kinase, 90kD, 1|ribosomal protein S6 kinase, 90kD, polypeptide 1
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Interactome
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Pathway
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Disease
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