SUMO1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate with SUMO1 recombinant monoclonal antibody (Cat # RAB02731) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using SUMO1 recombinant monoclonal antibody (Cat # RAB02731). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HeLa cells using SUMO1 recombinant monoclonal antibody (Cat # RAB02731)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against SUMO1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant SUMO1.
Theoretical MW (kDa)
12
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of SUMO1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate with SUMO1 recombinant monoclonal antibody (Cat # RAB02731) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using SUMO1 recombinant monoclonal antibody (Cat # RAB02731). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using SUMO1 recombinant monoclonal antibody (Cat # RAB02731)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — SUMO1
Entrez GeneID
7341Protein Accession#
P63165Gene Name
SUMO1
Gene Alias
DAP-1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, SUMO-1, UBL1
Gene Description
SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Omim ID
601912Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq
Other Designations
GAP modifying protein 1|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-like 1 (sentrin)
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Interactome
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Disease
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