SUMO1 monoclonal antibody (M03), clone 3D7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of SUMO1 expression in transfected 293T cell line by SUMO1 monoclonal antibody (M03), clone 3D7.
Lane 1: SUMO1 transfected lysate (Predicted MW: 11.6 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SUMO1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SUMO1 is 0.1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to SUMO1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (38 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant SUMO1.
Immunogen
SUMO1 (NP_001005781.1, 1 a.a. ~ 101 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (38 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SUMO1 expression in transfected 293T cell line by SUMO1 monoclonal antibody (M03), clone 3D7.
Lane 1: SUMO1 transfected lysate (Predicted MW: 11.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SUMO1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SUMO1 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SUMO1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — SUMO1
Entrez GeneID
7341GeneBank Accession#
NM_001005781.1Protein Accession#
NP_001005781.1Gene Name
SUMO1
Gene Alias
DAP-1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, SUMO-1, UBL1
Gene Description
SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Omim ID
601912Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq
Other Designations
GAP modifying protein 1|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-like 1 (sentrin)
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Interactome
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Disease
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