SUMO1 recombinant monoclonal antibody, clone 5G3
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More Files
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Immunohistochemistry
Immunohistochemical staining of human adrenal gland tissue with SUMO1 recombinant monoclonal antibody, clone 5G3 (Cat # RAB04327) (diluated at 1:92.5).
Immunohistochemistry
Immunohistochemical staining of human liver cancer with SUMO1 recombinant monoclonal antibody, clone 5G3 (Cat # RAB04327) (diluated at 1:92.5)
Immunoprecipitation
Immunoprecipitation analysis of HEK293T cell lysate with SUMO1 recombinant monoclonal antibody, clone 5G3 (Cat # RAB04327).
Lane 1: rabbit control IgG, Lane 2: RAB04327 precipitates and Lane 3: Input (HEK293T whole cell lysate).
Flow Cytometry
Flow cytometric analysis of HeLa cells with SUMO1 recombinant monoclonal antibody, clone 5G3 (Cat # RAB04327) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SUMO1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human SUMO1.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemical staining of human adrenal gland tissue with SUMO1 recombinant monoclonal antibody, clone 5G3 (Cat # RAB04327) (diluated at 1:92.5).Immunohistochemistry
Immunohistochemical staining of human liver cancer with SUMO1 recombinant monoclonal antibody, clone 5G3 (Cat # RAB04327) (diluated at 1:92.5)Immunoprecipitation
Immunoprecipitation analysis of HEK293T cell lysate with SUMO1 recombinant monoclonal antibody, clone 5G3 (Cat # RAB04327).
Lane 1: rabbit control IgG, Lane 2: RAB04327 precipitates and Lane 3: Input (HEK293T whole cell lysate).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with SUMO1 recombinant monoclonal antibody, clone 5G3 (Cat # RAB04327) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — SUMO1
Entrez GeneID
7341Protein Accession#
P63165Gene Name
SUMO1
Gene Alias
DAP-1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, SUMO-1, UBL1
Gene Description
SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Omim ID
601912Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq
Other Designations
GAP modifying protein 1|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-like 1 (sentrin)
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Interactome
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Disease
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