TOMM20 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TOMM20.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOMM20 (NP_055580.1).
Theoretical MW (kDa)
16
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of TOM20 protein.
Form
Liquid
Purification
Affinity chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:20-1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:MCF7 whole cell lysate Lane3:F9 whole cell lysate Lane4:PC12 whole cell lysate with TOMM20 recombinant monoclonal antibody (Cat # RAB02649) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using TOMM20 recombinant monoclonal antibody (Cat # RAB02649). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HepG2 cells using TOMM20 recombinant monoclonal antibody (Cat # RAB02649)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — TOMM20
Entrez GeneID
9804Protein Accession#
Q15388Gene Name
TOMM20
Gene Alias
KIAA0016, MAS20, MGC117367, MOM19, TOM20
Gene Description
translocase of outer mitochondrial membrane 20 homolog (yeast)
Omim ID
601848Gene Ontology
HyperlinkOther Designations
OTTHUMP00000037593|translocase of outer mitochondrial membrane 20 homolog|translocase of outer mitochondrial membrane 20 homolog type II
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