TOMM20 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TOMM20.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of human TOMM20.
Theoretical MW (kDa)
16
Reactivity
Hamster, Human
Specificity
Recognizes endogenous levels of TOM20 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela (A), CHOK1 (B) whole cell lysates with TOMM20 recombinant monoclonal antibody (Cat # RAB02548).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using TOMM20 recombinant monoclonal antibody (Cat # RAB02548). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.17). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with TOMM20 recombinant monoclonal antibody (Cat # RAB02548). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — TOMM20
Entrez GeneID
9804Protein Accession#
Q15388Gene Name
TOMM20
Gene Alias
KIAA0016, MAS20, MGC117367, MOM19, TOM20
Gene Description
translocase of outer mitochondrial membrane 20 homolog (yeast)
Omim ID
601848Gene Ontology
HyperlinkOther Designations
OTTHUMP00000037593|translocase of outer mitochondrial membrane 20 homolog|translocase of outer mitochondrial membrane 20 homolog type II
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