SMARCA2 recombinant monoclonal antibody, clone R02-3E2
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using SMARCA2/BRM antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunocytochemistry
Immunocytochemistry analysis of SMARCA2 (green) in 293 using SMARCA2 antibody,and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SMARCA2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against synthetic peptide corresponding to human SMARCA2/BRM.
Theoretical MW (kDa)
Calculated MW: 181 k
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using SMARCA2/BRM antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry analysis of SMARCA2 (green) in 293 using SMARCA2 antibody,and DAPI(blue).Immunofluorescence
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Gene Info — SMARCA2
Entrez GeneID
6595Protein Accession#
P51531Gene Name
SMARCA2
Gene Alias
BAF190, BRM, FLJ36757, MGC74511, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a
Gene Description
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Omim ID
600014Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq
Other Designations
OTTHUMP00000020970|OTTHUMP00000020971|SNF2-alpha|SNF2-like 2|SNF2/SWI2-like protein 2|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2|global transcription activator homologous sequence|sucrose nonfermenting 2-like protein 2
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Interactome
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Disease
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