Products

Western Blot (Cell lysate)

Western blot analysis of A549 (A), HL60 (B), U2OS (C) whole cell lysates with SMARCA2 recombinant monoclonal antibody (Cat # RAB02610).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using SMARCA2 recombinant monoclonal antibody (Cat # RAB02610). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.100). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunocytochemistry

Immunofluorescence

Immunofluorescent analysis of HEK293 cells with SMARCA2 recombinant monoclonal antibody (Cat # RAB02610). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Entrez GeneID

6595

Protein Accession#

P51531

Gene Name

SMARCA2

Gene Alias

BAF190, BRM, FLJ36757, MGC74511, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a

Gene Description

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2

Omim ID

600014

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq

Other Designations

OTTHUMP00000020970|OTTHUMP00000020971|SNF2-alpha|SNF2-like 2|SNF2/SWI2-like protein 2|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2|global transcription activator homologous sequence|sucrose nonfermenting 2-like protein 2